Development of an ELISA for the detection of the residues of the insecticide imidacloprid in agricultural and environmental samples.

نویسندگان

  • J K Lee
  • K C Ahn
  • O S Park
  • S Y Kang
  • B D Hammock
چکیده

A competitive enzyme-linked immunosorbent assay (ELISA) for the chloronicotinyl insecticide imidacloprid was developed using a polyclonal antibody produced against a hapten conjugated through the imidazolidine to keyhole limpet hemocyanin. In the standard curve of imidacloprid, an IC(50) of 17.3 ng/mL was obtained using a competitive heterologous system at pH 10. Very low cross-reactivity was found for some structurally related compounds including the insecticide thiacloprid. The high cross-reactivity with a metabolite containing the carbonyl group in the imidazolidine moiety suggests the involvement of its polarity and stereochemical fitness in forming the antibody--antigen complex. The effects of various assay conditions, including organic solvents, detergent content, salt concentration, and pH on the sensitivity were evaluated. High-performance liquid chromatography was run for comparison to validate the ELISA with fortified water samples, the correlation being 0.997-0.998 (n = 15) with a slope of 1.10--1.38. The ELISA turned out to be a convenient tool for monitoring imidacloprid residues in agricultural and environmental samples.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Food Safety Evaluation of Imidacloprid Residues in Grape Berries at a Different Dose of Spraying

Background & Aims of the Study: Grape, a crucial agriculture crop of Malayer city, is affected by Vine cicada, Psalmocharias alhageos.  Imidacloprid, a neonicotinoid insecticide, provides a good management of this insect. The aim of this research is residue persistence study of imidacloprid on grapes, to estimate its residue deposit, the half-life of degradation and safe pre-harvest consumption...

متن کامل

Detection of quinolones residues in beef and chicken meat in hypermarkets of Urmia, Iran using ELISA

ABSTRACT- The aim of this study was to determine the quinolone residues in beef and chicken meat samples collected from Urmia region local markets.A total of 395 beef and chicken meat samples varied in three various brands were analyzed in Urmia, Iran by using a sensitive and reliable analytic method based on ELISA detection, for quantification of quinolone antibiotics residues. Samples prepara...

متن کامل

اندازه‌گیری باقیمانده و دوره کارنس حشره‌کش ایمیداکلوپرید روی خیار گلخانه‌ای در منطقه ورامین

Extensive use of chemical pesticides to control pests in developed and developing countries has led to the increase in crop production and decrease in post-harvest losses, which has caused harmful effects on human health. When the amount of pesticides exceeds permissible limits, some measures should be undertaken to reduce their application. In order to control cucumber pests in greenhouse, far...

متن کامل

Determination of malachite green and leucomalachite green residues in rainbow trout in Sanandaj City

Background and Aim: Malachite green (MG) is an organic compound which his used as a dye in textile industry. This compound is applied incorrectly and illegally in aquiculture as an antimicrobial agent. This compound has mutagenic and carcinogenic effects. Materials and Methods: In this study we determined the concentrations of MG and leucomalachite green (LMG) in the muscle tissue of 90 rainbow...

متن کامل

Removal of imidacloprid insecticide in the photocatalytic activation of proxymonosulfate with modified magnesium oxide as catalyst using ultraviolet A

Background and Objective: Imidacloprid, a neonicotinide plant toxin, is used as an insecticide in agriculture. Due to its high degradation resistance and water solubility it is of highly concerns. Therefore, the aim of this study was to investigate the degradation of imidacloprid by modified magnesium oxide catalyst under irradiation of light and peroxymonosulfate. Materials and Methods: In th...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of agricultural and food chemistry

دوره 49 5  شماره 

صفحات  -

تاریخ انتشار 2001